| 1 | Lashinda Demus | USA | |
52,47 | AR |
| 2 | Melaine Walker | JAM | |
52,73 | |
| 3 | Natalya Antyukh | RUS | |
53,85 | |
| 4 | Kaliese Spencer | JAM | 54,01 | ||
| 5 | Anastasiya Rabchenyuk | UKR | 54,18 | ||
| 6 | Vania Stambolova | BUL | 54,23 | ||
| 7 | Zuzana Hejnová | CZE | 54,23 | ||
| 8 | Elena Churakova | RUS | 55,17 |