| 1 | Amantle Montsho | BOT | |
49,56 | NR |
| 2 | Allyson Felix | USA | |
49,59 | |
| 3 | Anastasiya Kapachinskaya | RUS | |
50,24 | |
| 4 | Francena McCorory | USA | 50,45 | ||
| 5 | Antonina Krivoshapka | RUS | 50,66 | ||
| 6 | Shericka Williams | JAM | 50,79 | ||
| 7 | Sanya Richards-Ross | USA | 51,32 | ||
| 8 | Novlene Williams-Mills | JAM | 52,89 |