| 1 | Jamaika | JAM | 41,88 | Q | |
| (Simone Facey, Shelly-Ann Fraser, | |||||
| Aleen Bailey, Kerron Stewart) | |||||
| 2 | Bahamas | BAH | 42,66 | Q | |
| (Sheniqua Ferguson, Chandra Sturrup, | |||||
| Christine Amertil, Debbie Ferguson-McKenzie) | |||||
| 3 | Trinidad & Tobago | TRI | 43,22 | q | NR |
| (Reyare Thomas, Kelly-Ann Baptiste, | |||||
| Ayanna Hutchinson, Semoy Hackett) | |||||
| 4 | Ukraine | UKR | 43,77 | ||
| (Olena Chebanu, Nataliya Pohrebnyak, | |||||
| Mariya Ryemyen, Hrystyna Stuy) | |||||
| 5 | Saint Kitts & Nevis | SKN | 43,98 | ||
| (Tanika Liburd, Meritzer Williams, | |||||
| Tameka Williams, Virgil Hodge) | |||||
| 6 | Belgien | BEL | 43,99 | ||
| (Olivia Borlee, Hanna Mariën, | |||||
| Elodie Ouédraogo, Anne Zagre) |
| 1 | Deutschland | GER | 42,96 | Q | |
| (Marion Wagner, Anne Möllinger, | |||||
| Cathleen Tschirch, Verena Sailer) | |||||
| 2 | Kolumbien | COL | 43,30 | Q | |
| (Yomara Hinestroza, Felipa Palacios, | |||||
| Darlenis Obregón, Norma González) | |||||
| 3 | Großbritannien | GBR | 43,34 | q | |
| (Laura Turner, Montell Douglas, | |||||
| Emily Freeman, Emma Ania) | |||||
| 4 | Weißrußland | BLR | 44,12 | ||
| (Hanna Bahdanovich, Aksana Drahun, | |||||
| Alena Kievich, Yulia Nestsiarenka) | |||||
| 5 | Thailand | THA | 44,59 | ||
| (Sangwan Jaksunin, Orranut Klomdee, | |||||
| Jutamass Tawoncharoen, Jintara Seangdee) | |||||
| 6 | Nigeria | NGR | 46,54 | ||
| (Olutoyin Augustus, Halimat Ismaila, | |||||
| Seun Adigun, Oludamola Osayomi) |
| 1 | Brasilien | BRA | 43,07 | Q | |
| (Rosemar Maria Neto, Lucimar Aparecida de Moura, | |||||
| Thaissa Presti, Vanda Gomes) | |||||
| 2 | Rußland | RUS | 43,18 | Q | |
| (Evgeniya Polyakova, Aleksandra Fedoriva, | |||||
| Yulia Gushchina, Natalia Rusakova) | |||||
| 3 | Polen | POL | 43,63 | ||
| (Iwona Ziòlkowska, Marta Jeschke, | |||||
| Dorota Jedrusinska, Iwona Brzezinska) | |||||
| 4 | Japan | JPN | 44,24 | ||
| (Chisato Fukushima, Momoko Takahashi, | |||||
| Mayumi Watanabe, Maki Wada) | |||||
| USA | USA | DNF | |||
| (Lauryn Williams, Alexandria Anderson, | |||||
| Muna Lee, Carmelita Jeter) |