| 1 | Melaine Walker | JAM | |
52,42 | AR |
| 2 | Lashinda Demus | USA | |
52,96 | |
| 3 | Josanne Lucas | TRI | |
53,20 | NR |
| 4 | Kaliese Spencer | JAM | 53,56 | ||
| 5 | Tiffany Williams | USA | 53,83 | ||
| 6 | Natalya Antyukh | RUS | 54,11 | ||
| 7 | Anastasiya Rabchenyuk | UKR | 54,78 | ||
| 8 | Angela Morosanu | ROU | 55,04 |