| 1 | Sanya Richards | USA | |
49,00 |
| 2 | Shericka Williams | JAM | |
49,32 |
| 3 | Antonina Krivoshapka | RUS | |
49,71 |
| 4 | Novlene Williams-Mills | JAM | 49,77 | |
| 5 | Christine Ohuruogu | GBR | 50,21 | |
| 6 | Debbie Dunn | USA | 50,35 | |
| 7 | Anastasiya Kapachinskaya | RUS | 50,53 | |
| 8 | Amantle Montsho | BOT | 50,65 |