| 1 | Tonique Williams-Darling | BAH | |
49,55 |
| 2 | Sanya Richards | USA | |
49,74 |
| 3 | Ana Guevara | MEX | |
49,81 |
| 4 | Svetlana Pospelova | RUS | 50,11 | |
| 5 | DeeDee Trotter | USA | 51,14 | |
| 6 | Olesya Zykina | RUS | 51,24 | |
| 7 | Monique Henderson | USA | 51,77 | |
| 8 | Amy Mbacke Thiam | SEN | 52,22 |