| 1 | Yuliya Pechonkina | RUS | 54,01 | Q |
| 2 | Tetiana Tereschuk-Antipova | UKR | 55,28 | Q |
| 3 | Joanna Hayes | USA | 56,10 | Q |
| 4 | Androula Sialou | CYP | 56,11 | |
| 5 | Rebecca Wardell | NZL | 56,51 | |
| 6 | Monika Niederstatter | ITA | 56,62 | |
| 7 | Salhate Djamaldine | COM | 58,91 |
| 1 | Surita Febbraio | RSA | 55,41 | Q |
| 2 | Heike Meissner | GER | 55,80 | Q |
| 3 | Natalya Torshina | KAZ | 55,86 | Q |
| 4 | Natasha Danvers | GBR | 56,02 | q |
| 5 | Andrea Blackett | BAR | 56,05 | q |
| 6 | Mame Tacko Diouf | SEN | 56,22 | |
| 7 | Jessica Miller | URU | 1:01,03 | |
| 8 | Diala El Chab | LIB | 1:02,90 |
| 1 | Jana Pittman | AUS | 54,80 | Q |
| 2 | Raasin McIntosh | USA | 55,53 | Q |
| 3 | Stephanie Kampf | GER | 55,79 | Q |
| 4 | Anna Jesien | POL | 56,07 | |
| 5 | Ieva Zunda | LAT | 56,87 | |
| 6 | Velveth Moreno | PAN | 1:03,55 | |
| Yvonne Harrison | PUR | DNF |
| 1 | Sandra Glover | USA | 54,48 | Q |
| 2 | Ionela Tirlea | ROM | 54,98 | Q |
| 3 | Malgorzata Pskit | POL | 55,18 | Q |
| 4 | Allison Beckford | JAM | 55,75 | q |
| 5 | Cora Olivero | ESP | 55,95 | q |
| 6 | Makiko Yoshida | JPN | 57,08 | |
| 7 | Aïssata Soulama | BUR | 1:01,86 |
| 1 | Jana Pittman | AUS | 53,77 | Q |
| 2 | Sandra Glover | USA | 53,90 | Q |
| 3 | Tetiana Tereschuk-Antipova | UKR | 54,26 | Q |
| 4 | Heike Meissner | GER | 55,00 | Q |
| 5 | Natalya Torshina | KAZ | 55,24 | |
| 6 | Joanna Hayes | USA | 55,35 | |
| 7 | Natasha Danvers | GBR | 55,48 | |
| 8 | Cora Olivero | ESP | 55,82 |
| 1 | Yuliya Pechonkina | RUS | 53,57 | Q |
| 2 | Ionela Tirlea | ROM | 54,84 | Q |
| 3 | Surita Febbraio | RSA | 55,18 | Q |
| 4 | Andrea Blackett | BAR | 55,33 | Q |
| 5 | Stephanie Kampf | GER | 55,35 | |
| 6 | Allison Beckford | JAM | 55,76 | |
| 7 | Malgorzata Pskit | POL | 56,21 | |
| Raasin McIntosh | USA | DNF |