| 1 | Michael Johnson | USA | |
43,18 | WR |
| 2 | Sanderlei Claro Parrela | BRA | |
44,29 | AR |
| 3 | Alejandro Cárdenas | MEX | |
44,31 | NR |
| 4 | Antonio Pettigrew | USA | 44,54 | ||
| 5 | Mark Richardson | GBR | 44,65 | ||
| 6 | Gregory Haughton | JAM | 45,07 | ||
| 7 | Jamie Baulch | GBR | 45,18 | ||
| Jerome Young | USA | DQ |