| 1 | Marie-José Pérec | FRA | |
49,28 | |
| 2 | Pauline Davis-Thompson | BAH | |
49,96 | NR |
| 3 | Jearl Miles-Clark | USA | |
50,00 | |
| 4 | Cathy Freeman | AUS | 50,60 | ||
| 5 | Fatima Yusuf | NGR | 50,70 | ||
| 6 | Falilat Ogunkoya | NGR | 50,77 | ||
| 7 | Maicel Malone-Wallace | USA | 50,99 | ||
| 8 | Sandie Richards | JAM | 51,13 |