| 1 | Marie-José Pérec | FRA | |
49,13 | NR |
| 2 | Grit Breuer | GER | |
49,42 | |
| 3 | Sandra Myers | ESP | |
49,78 | |
| 4 | Olga Vladykina-Bryzgina | URS | 49,82 | ||
| 5 | Jearl Miles-Clark | USA | 50,50 | ||
| 6 | Ximena Restrepo | COL | 50,79 | ||
| 7 | Lillie Leatherwood | USA | 51,53 | ||
| 8 | Diane Dixon | USA | 51,73 |