| 1 | Alleyne Francique | GRN | 45,86 | Q |
| 2 | California Molefe | BOT | 46,02 | Q |
| 3 | Milton Campbell | USA | 46,22 | Q |
| 4 | Daniel Dabrowski | POL | 46,61 | |
| 5 | Ruwen Faller | GER | 46,67 | |
| 6 | Vladislav Frolov | RUS | 47,11 |
| 1 | Christopher Brown | BAH | 46,10 | Q |
| 2 | Davian Clarke | JAM | 46,23 | Q |
| 3 | Dmitriy Petrov | RUS | 46,44 | Q |
| 4 | Marcin Marciniszyn | POL | 46,97 | |
| 5 | Carlos Santa | DOM | 47,11 | |
| 6 | David McCarthy | IRL | 47,11 |