| 1 | California Molefe | BOT | 45,74 | Q | |
| 2 | Dmitriy Petrov | RUS | 46,29 | Q | |
| 3 | Alexey Rachkovskiy | UKR | 47,13 | ||
| 4 | Santiago Ezquerro | ESP | 47,36 | ||
| 5 | David Gillick | IRL | 47,61 | ||
| 6 | Chon Kin Sou | MAC | 49,95 | NR |
| 1 | Alleyne Francique | GRN | 46,79 | Q |
| 2 | Marcin Marciniszyn | POL | 46,88 | Q |
| 3 | Ruwen Faller | GER | 47,02 | q |
| 4 | Valentin Bulichev | AZE | 48,26 | |
| Sanjay Ayre | JAM | DQ |
| 1 | Christopher Brown | BAH | 46,64 | Q | |
| 2 | Vladislav Frolov | RUS | 46,69 | Q | |
| 3 | Carlos Santa | DOM | 47,00 | q | |
| 4 | Isaac Yaya | PYF | 48,45 | NR | |
| Asad Iqbal | PAK | DQ |
| 1 | Milton Campbell | USA | 46,67 | Q | |
| 2 | Daniel Dabrowski | POL | 46,80 | Q | |
| 3 | Arismendy Peguero | DOM | 47,32 | ||
| 4 | Chris Lloyd | DMA | 47,33 | ||
| 5 | Andrés Silva | URU | 47,50 | NR | |
| 6 | Brice Panel | FRA | 48,52 |
| 1 | Davian Clarke | JAM | 46,37 | Q | |
| 2 | David McCarthy | IRL | 46,68 | Q | |
| 3 | David Canal | ESP | 47,38 | ||
| 4 | Anderson Jorge dos Santos | BRA | 48,19 | ||
| 5 | Nicholas Mangham | PLW | 54,80 | NR | |
| LaShawn Merritt | USA | DQ |