| 1 | Vanya Stambolova | BUL | 50,66 | Q |
| 2 | Natalya Nazarova | RUS | 50,67 | Q |
| 3 | Novlene Williams | JAM | 51,25 | Q |
| 4 | Ilona Usovich | BLR | 51,53 | |
| 5 | Claudia Hoffmann | GER | 52,32 | |
| 6 | Grazyna Prokopek | POL | 53,64 |
| 1 | Olesya Krasnomovez | RUS | 50,61 | Q |
| 2 | Christine Amertil | BAH | 51,09 | Q |
| 3 | Mariyana Dimiotrova | BUL | 52,17 | Q |
| 4 | Sanya Richards | USA | 52,46 | |
| 5 | Angela Morosanu | ROM | 52,57 | |
| 6 | Monika Bejnar | POL | 52,60 |